Fastest commercially available tissue clearing method
Preserves tissue morphology and target fluorescence.
No need for organic solvents.
Binaree Tissue Clearing Rapid offers a very fast process. For example, mice brains can be cleared in just 6 hours. Unlike the methods used in the current tissue clearing market, Binaree provides morphological consistency while preserving existing fluorescence as it utilizes electrophoresis and mild chemicals. It does not use heat or organic solvents while keeping the clean-up capacity of lipids. Additionally, Binaree is compatible with downstream immunostaining.
Fig. 1 Comparison of marketed products (Ref. www.Binaree.com)
Outlined protocol of tissue clearing with Binaree Tissue Clearing Rapid™.
– Example of mouse brain clearing.
- Prepare a transcardial perfused sample with 4 % paraformaldehyde (PFA).
- Incubate the sample with 4 % Paraformaldehyde PFA overnight.
- Incubate in 30 % sucrose solution until the sample sinks.
- Set tissue in the chamber, fill with Binaree Tissue Clearing Rapid Solution and place the chamber into the electrophoresis kit, run only for 6 to 8 hours in the case of mouse brain.
- Incubate in Binaree Tissue Clearing Rapid Mounting Solution at 37 °C for 24-48 hours.
The detailed protocol of Tissue Clearing Rapid can be downloaded.
Watch Binaree® Tissue Clearing Rapid™ in action!
What is Tissue Clearing
Tissue-clearing technology makes tissue transparent, allowing you to visualize opaque organs and tissues in three dimensions.
High-resolution mapping of three-dimensional (3D) imaging of intact brains has been used as a viable approach to visualizing neuroanatomical data. Specifically, these imaging techniques enable the spatial localization and overall morphological observation of target cells within tissues, thereby expanding the scope of biological and medical insights beyond traditional, labor-intensive sectioning methods to encompass holistic information. The recently developed tissue-clearing techniques combine with optical imaging equipment such as confocal and light-sheet microscopy and imaging programs to enable the identification of biological information in 3D directly without the need to collate 2D images.
How Binaree Tissue Clearing Rapid works
Binaree Tissue Clearing Rapid™ works to lower the refractive index (RI) of cell and tissue components. As a result, the treated tissue becomes low viscosity and optically clear. It gives a refractive index of 1.52 which requires no additional processing prior to imaging. When compared to a commonly used detergent, Triton-X, the micelles produced by the gentle detergent in Binaree Rapid are significantly smaller and more permeable to tissue.
When paring the Tissue Clearing Rapid with our electrophoresis device, the tissue clearing step can be completed in the following timeframes:
With the Binaree® Tissue Clearing Rapid™ system the whole clearing process from fixation to imaging takes approximately 2-3 days, which is significantly faster than many other available tissue clearing methods. Options for passive clearing without the need for the electrophoresis device are available and this process takes approximately 5-7 days to achieve tissue clearing.
Publications
Please visit this page https://www.binaree.com/tissue-clearing-reference
Binaree® Tissue Clearing Rapid™ System includes (BDTC-003 , B00.003.00)
Electrophoresis Device (BDTC-003)
The BDTC-003, electrophoresis device has a cooling function to keep the sample temperature below 30°C to prevent tissue from a fluorescence loss.
- Full Digital LCD Display with Black-Light Function
- 165 x 250 x 145 mm, 2.2 kg
- Digital Fuzzy Controller with Touch Button and Job Dial
- Timer 99 hr 59 min, 1 min increments
For first-time use, we would recommend purchasing;
Rapid System (BDTC-003) + Rapid Solution (BRTC-001 or BRTC-002) + Rapid Mounting (BRMO-006)
Binaree® Tissue Clearing Rapid™ Reagents for Brain
| Product Code | Product Name | Description | Volume |
|---|---|---|---|
| B10.001.00 | BRTC-001 | Binaree Tissue Clearing Rapid Solution for 3 mouse brain samples | 60 mL |
| B10.101.00 | BRMO-006 | Binaree Tissue Clearing Rapid Mounting Solution for 3 mouse brain samples | 60 mL |
| B10.401.00 | BRTC-401 | Binaree Tissue Clearing Rapid Solution Brain for 30 mouse brains samples | 600 mL |
| B12.060.00 | BRMO-060 | Binaree Tissue Clearing Rapid Mounting Solution for 30 mouse brain samples | 600 mL |
Binaree® Tissue Clearing Rapid™ Reagents (non-Brain)
| Product Code | Product Name | Description | Volume |
|---|---|---|---|
| B11.002.00 | BRTC-002 | Binaree Tissue Clearing Rapid Solution for 3 samples | 60 mL |
| B11.402.00 | BRTC-402 | Binaree Tissue Clearing Rapid Solution x10 | 600 mL |
Remark: Rapid system and reagents do not work with passive type reagents e.g. HRTC-xxx, SHMS-xxx, HRMO-xxx, etc.
Binaree® Passive Tissue Clearing Reagents
| Product Code | Product Name | Description | Volume |
|---|---|---|---|
| B20.012.00 | HRTC-012 | Binaree Tissue Clearing Kit | 30 mL of Solution A 60 mL of Solution B |
| B20.312.00 | HRTC-312 | Binaree Tissue Clearing Kit for 5 brain samples | 150 mL of Solution A 300 mL of Solution B |
| B20.412.00 | HRTC-412 | Binaree Tissue Clearing Kit for 10 brain samples | 300 mL of Solution A 600 mL of Solution B |
| B21.006.00 | HRMO-006 | Binaree Tissue Clearing Mounting Solution for 3 brain samples | 60 mL |
| B21.030.00 | HRMO-030 | Binaree Tissue Clearing Mounting Solution for 15 brain samples | 300 mL |
| B21.060.00 | HRMO-060 | Binaree Tissue Clearing Mounting Solution for 30 brain samples | 600 mL |
***Passive
- Clearing the adult mouse whole brain at 5-7 days
- Clearing only with solution, no electrophoresis device
- Recommended for tissue clearing under 3 mm
- Immunostaining possible after clearing.