Last Updated on September 20, 2023
Here’s why we recommend HPLC-ECD for neurotransmitter detection.
While there are a few ways to analyze neurotransmitters, like liquid chromatography-mass spectrometry, or LC-MS, ELISA, or Radioimmunoassays. However, for qualitative and quantitative analysis of neurotransmitters, we recommend high-performance liquid chromatography detected with an electrochemical detector, known as HPLC-ECD.
The first reason is sensitivity. HPLC-ECD is highly sensitive, down to the femtomolar range. This is suitable for most neuroscience applications, including a quantitative analysis of in-vivo microdialysis samples.
The second reason is time. Applications using HPLC-ECD can run anywhere from 5 to 30 minutes, where you can detect multiple analytes from a single injection. For example, we have an application for the simultaneous detection of dopamine and serotonin in just 5 minutes. That means you can run over a hundred samples overnight for high throughput using laboratory automation like an autosampler. We’ve also developed applications, saving you the economy of time by already optimizing conditions. All the applications on our site we know work, because we have tested them in-house.
The third reason is price. HPLC-ECD is priced with intended use in your own lab, thus, no need to outsource analysis in another lab or core facility. This gives you, the user, more confidence in your data knowing that you are in control. Pricing for the equipment typically ranges from $20,000 to $60,000. If you’re not quite ready to commit to a new machine, ask us about our leasing options.
Finally, usability. While HPLC can seem overwhelming and complicated, we’ve simplified it with the HTEC. The HTEC system is an all-in-one device that integrates everything you need into one instrument. It’s straightforward and easy to use. Should you decide to acquire one of our instruments, on-site installation and training at your lab will be provided. Once installed, you can immediately start running samples. If you have questions or need assistance, there’s no need to worry. Our self-help support site and knowledgeable support staff are available to guide you through the process, including how to use it as well as help with troubleshooting. At Amuza, it’s important for us that our instruments work just as well for you in your hands as they do for us.
Therefore, we recommend HPLC-ECD for the detection of neurotransmitters.
For more information, contact us today.
Im interested in analysing mouse brain dopamine metabolites
Can you provide a quote for your system?
Thank you for your interest Joanne! The HTEC-510 paired with our SC-5ODS column works very well to quantitate monoamines and their metabolites; you can find information about this application here.
Green Leaf Scientific, located in Ireland, is our distributor for neuroscience products in Europe and will be happy to prepare your quote for you.
Have you used this system to quantify GABA in the nM range?
Hello Michael,
Thank you for reaching out! Our HPLC-ECD is capable of detecting the nM range of GABA. We would be happy to provide more detailed information. What is your target sample type?