Last Updated on August 24, 2023

Benefits of using the ENO-30 for Nitrate and Nitrite Detection

Today, I’d like to talk about a system capable of quantitative analysis of nitrate and nitrite from biological samples in minutes, called the ENO-30. The ENO-30 is a fast and highly sensitive system down to 0.1 picomoles.

Let me walk you through how easy it is to use. First, you get your sample ready for analysis. Sample prep is easy. In our manual, we’ve outlined a simple sample preparation protocol for tissue homogenate, blood, cell culture, urine, saliva, and microdialysis samples. Samples are injected into the ENO-30 with a manual injector or an autosampler for laboratory automation.

Once injected, nitrate and nitrite are separated by an HPLC separation column. Once separated, nitrate is reduced to nitrite, forming 2 separated nitrite compounds. Then, the separated nitrites are mixed with a Greiss reagent to form an azo dye, which is then detected by absorbance at 540 nm. In 10 minutes, you will be able to detect both nitrate and nitrite from a single injection.

The ENO-30 combines HPLC with colorimetric analysis. It’s like having a chemist and HPLC expert right on your benchtop. We’ve optimized the conditions to take away any guesswork and made the system straightforward and easy to use.

At Amuza, your success is our priority. It’s important that our products work for you just as well as they do for us here at our facility. When you acquire one of our machines, not only will it include installation and training, you’ll have access to our self-help support center as well as support from our knowledgeable support staff.