In this video, we’ll show you how to detect and analyze Glutamate and GABA in brain tissue or microdialysis samples in just 12 minutes. For this analysis, we’ll be derivatizing our analytes with OPA and beta-mercaptoethanol and analyze with high-performance liquid chromatography or HPLC, coupled with an electrochemical detector. But don’t let any of that scare you. We’ve simplified and automated the process to make it easier than ever.
The generation of good data and consistent results can take time and resources. That’s why many labs require highly-trained specialists in HPLC. However, we’ve created an application, which includes everything you need to run analyses right away. Everything from the mobile phase, reagent, and column selection, we have everything spelled out for you so all you have to do is take your sample and start running analysis. We test everything in house to make sure it works, ensuring there are no surprises.
For this application, we recommend use with our autosampler, called the AS-700. This enables proper mixing and incubation times, which provide consistent results sample after sample. Even with the precolumn derivatization process, It’s fast and highly sensitive, down to 1 picomole for glutamate, and 100 femtomoles for GABA.
At Amuza, we always have the end-user in mind. That’s why when you purchase one of our machines, you also have access to our self-help support center as well as our support specialists that can assist you with troubleshooting or replacement of parts as needed.
For more information on glutamate and GABA analysis, contact us today!