Eicom HPLC-ECD Applications
Developed and Tested at Eicom. We can provide technical support for all of our analytical applications because they were developed and tested extensively in Eicom’s lab.
Step-by-step protocols. The application notes that are available on this page highlight each method. More detailed step-by-step protocols are provided to our customers.
Easy to switch between methods. If you need to use multiple methods on the same system, Eicom systems can easily be switched over because they have such a small, internal dead volume (thanks to the elimination of a pulse damper), and detector cells that stabilize quickly.
Application manuals and other documents can be downloaded for free from our support site. Login may be required.
Microdialysis Sample Analysis
Highly Sensitive Acetylcholine Analysis – Cholinesterase Inhibitor Free
- No acetylcholinesterase inhibitor required for the medial prefrontal cortex.
- The new improved enzymatic column provides increased sensitivity
- The reproducible sensitivity level of 5 fmol
- 18 minute run time with an internal standard, 13 minutes without internal standard.
- Also allows for the detection of Choline
Ultra Sensitive Analysis of Dopamine and Serotonin in 5 Min
- Detection limit 160 Amol (0.16 fmol, 30 fg) for both DA and 5-HT
- Robust 5-minute analytical method
- Clear separation of peaks
The Simultaneous Analysis of Norepinephrine, Dopamine and Serotonin
- Simultaneous analysis in 15 minutes
- 260 Amol (50 fg) detection limit for DA and 580 Amol (0.1 pg) for NE and 5-HT
- Allows for higher sample temporal resolution (15 min)
*Find the best catecholamine analysis for your samples here.
Rapid GABA Analysis by o-phthaldialdehyde Derivatization
- Sensitivity level 30 fmol (1 nM x 30 µl).
- High specificity. Ca2+ free perfusate decreases the peak height.
- A 28-minute method with the incorporation of a switching valve > chromatogram
Glutamate and GABA Analysis in 12 Minutes
- A flexible analytical method with adaptability to microdialysate and tissue homogenate
- No UHPLC or two systems required.
Concise Glutamate Analysis by Using an Online Enzymatic Reaction
- No precolumn derivatization required
- A flexible analytical method with adaptability to microdialysate, blood, and tissue homogenate
- Microdialysis sample run time of 10 minutes
- The robust detection limit (< 3 fmol). (Microdialysis samples have higher concentrations) > chromatogram
Other Sample Analysis (Urine, Serum/Plasma, Tissue Homogenate)
Monoamines and Metabolites in Tissue Homogenate
- 11 peaks in 24 minutes
- Analyze MHPG, NE, Epi, DOPAC, NM, DA, 5-HIAA, ISO, HVA, 3MT, 5-HT in one injection
- Special condition for detection of L-DOPA in addition to above peaks (30 minute run time)
- Highly sensitive down to 0.5 fmol
Catecholamine Analysis in Plasma or Urine
- Approx. 0.5 fmol (=0.1 pg) sensitivity. 20 min for one sample > chromatogram
- Require sample preparation with activated alumina
* Find the best catecholamine analysis for your samples here
Analysis of Quinones such as CoQ10 and Vitamin K
- No platinum black is required
- Higher reproducibility
- Lower running cost
- We also have an application using platinum blacks.
Glutamate and GABA Analysis in 12 Minutes
- A flexible analytical method with adaptability to microdialysate and tissue homogenate
- No UHPLC or two systems required.
Concise Glutamate Analysis by Using an Online Enzymatic Reaction
- No precolumn derivatization required
- A flexible analytical method with adaptability to microdialysate, blood, and tissue homogenate
- Microdialysis sample run time of 10 minutes
- A robust detection limit (< 3 fmol). (Microdialysis samples have higher concentrations) > chromatogram
High Sensitive Thiols Detection using a Gold Working Electrode
- New – ECD with a gold working electrode
- Highly sensitive with 1 fmol detection limit for GSH and 0.5 fmol for -SH compounds > chromatogram
Detection of 3-Nitrotyrosine in Atmospheric Environments
- For particulate matter (PM) and total suspended particles (TSP) sample analysis
- Developed at Okayama University, Japan