Ultra-High Sensitivity Electrochemical Detection
Trust in the sensitivity of electrochemical detection. Rely on our pre-configured applications and system to easily ensure 160 attomole sensitivity for dopamine and serotonin, and 5 fmol for acetylcholine.
Turn Key System
Get started with high-throughput electrochemical detection right away with the all-in-one HPLC-ECD system. Confidently trust in our prepared neuroscience-specific analytical applications to get you the results you deserve. Simply choose the method and follow the detailed protocol.
Avoid the concern normally associated with system maintenance. The simple compact design of the HTEC-510 (HPLC-ECD) allows for easy maintenance. To further support you, we will be here every step of the way to make sure your system is always fully functional.
Superior Customer Support
Learning a new system can be stressful. We get it. That is why we make sure you gain the support you need. A trained application specialist will work with you directly from the beginning to make sure your experience is smooth and stress-free.
*The HTEC-600 replaces the HTEC-510*
Free Dopamine Analysis Application Note
Levels of dopamine and serotonin in microdialysate can widely vary, and sometimes the level can be as low as the nM level.
In this application note, we introduce a fast and ultra-sensitive method for analyzing dopamine and serotonin levels with improved throughput and resolution time.
Eicom’s Innovative Cell Design
- Ultrasensitive, 30 fg of dopamine
- Graphite electrode often ready to use for < 30 mins after start-up
- No tools to access the working electrode
- Easy to clean or change the working electrode
The key to the sensitivity is the cell design. Eicom relies on a spring-loaded mechanism that always maintains the proper pressure. This structure allows the detector to quickly stabilize after the cell is opened and closed. Another benefit is that the working electrode can easily be accessed with no tools. In this way, the electrode can easily be wiped clean and reinstalled to ensure maximum sensitivity.
The [HTEC-510] has been fantastic, it’s run beautifully, and it’s super easy to use.
I like that all of my settings can be controlled right here on the screen, instead of having to make method files within the software and uploading them to the system – that’s just too much work.
Major Reasons of Ultra-High Sensitivity and Stability of Eicom’s ECD
- Innovative Design of Electrochemical Detector Cell
- System Design with Pulsation Free Pump
All-In-One With A Small Footprint
A common sentiment of our users the first time they see our system is, “Is this a complete HPLC-ECD system?
It is so compact!” The HTEC-510 users are always pleasantly surprised with the small footprint. The HTEC-510 includes:
- An online degasser (only 300 µl volume)
- HPLC pump (no pulsation damper)
- Manual injector
- Column temperature controller (both cooling and heating)
- Electrochemical detector cell
Within a single package the size of a typical desktop computer. The trimmed HPLC-ECD sacrificed nothing in order to combine the latest technology into this concise unit, which has enhanced stability. This system actually has higher sensitivity levels than the preceding models, as well as the compact design. Read on to learn the latest technological advancements within the compact design.
Anatomy of the HTEC-510
No Pulse Damper Required
Normal HPLC pumps are not ideal for electrochemical detection. The EC detector is sensitive enough to detect pulsation from the pump and therefore, a pulse damper is required to reduce this noise. However, the pulse damper itself can cause problems. It requires over 10 mL to replace the inside volume. This volume can contribute to contamination following long periods of storage or shipping, and can be a serious problem for detection. The pulse damper needs to be filled completely with the mobile phase in order for the pressure to stabilize. It takes over 5 minutes. If any old mobile phase remained in the system and entered the new column, the upcoming analysis could be ruined. The presence of the pulse damper in the HPLC system was not ideal. So we removed the damper from the system and adopted a 4 µL replacement volume pump. This small binary pump has a computerized pulsation quenching technology. Once you start the pump, the pressure becomes stable in several seconds, and the piston-stroke speed changes during each stroke in order to quench pulsation. You can see the spindle-shaped baseline noise will converge to a thin flat line within one minute. The dead volume between the piston and the column is less than 1 ml, making cleaning the line quick and easy.
Directly Attain the Results
We understand you are looking for the best system by investigating a few key aspects of the systems, but is that all you need? You need the RESULTS got by the system. Eicom offers a turnkey solution and you can depend on us to get you the data. By telling us the target compounds and type of samples, Eicom will provide dependable application solutions including detailed application manuals, specially designed columns for the ECD, and technical support. You do not need to take time creating analytical methods or washing the column for a few days prior to kick off a new project. As long as you use Eicom’s HPLC-ECD and our analytical applications, the data will be easily generated. If you’ve never used an ECD or HPLC, starting with the most user-friendly machine is the best way to efficiently achieve your goals.
Summary and Additional Features of the HTEC-510
- Minimal maintenance and more uptime
- Small footprint
- Intuitive operation with an integrated control panel
- The system has inert materials at the liquid contacting surfaces which prevents metal ions from flowing to the detector that can result in noise
- Powerful applications.
- Excellent support
|Electrochemical Cell||Amperometric with 3 electrode DC potentiostat (standard)|
|Second Cell (optional)||Coulometric Cell|
|Working Electrode||Graphite composite (standard), glassy carbon, pure graphite, platinum, gold, silver|
|Counter Electrode||316 stainless steel|
|Applied Voltage Range||-2000 mV to +2000 mV, 1 mV increments|
|Auto Zero Function||Between -1000 nA and +1000 nA; triggered by external input|
|MicroFlow Pump||100-750 µL/min, 1 µL increments, 4µL/stroke, 20 MPa maximum pressure|
|Solvent Degasser||2 channels, 300 µL, and 7.5 mL|
|Temperature Range||15-55°C, 1°C increment; 20-45°C (recommended range)|
|Size (H x W x D)||25 x 39.9 x 36.5 cm|
The specifications are subject to change without notice.