HPLC-ECD, HTEC-600
Confidently Bring an All-In-One Electrochemical Detection System to Your Lab
Ultra-High Sensitivity Electrochemical Detection
Trust in the sensitivity of electrochemical detection. Rely on our pre-configured applications and system to easily ensure 160 attomole sensitivity for dopamine and serotonin, and 5 fmol for acetylcholine.
Turn Key System
Get started with high-throughput electrochemical detection right away with the all-in-one HPLC-ECD system. Confidently trust in our prepared neuroscience-specific analytical applications to get you the results you deserve. Simply choose the method and follow the detailed protocol.
Worry-free Maintenance
Avoid the concern normally associated with system maintenance. The simple compact design of the HTEC-600 (HPLC-ECD) allows for easy maintenance.
15 min Analysis
Obtain highly sensitive analysis of norepinephrine, dopamine & serotonin in microdialysis samples in 15 minutes.
To ensure you maintain the high sensitivity you deserve, we will be here every step of the way to make sure your system is always fully functional.
Superior Customer Support
Learning a new system can be stressful. We get it. That is why we make sure you gain the support you need. A trained specialist will work with you directly from the beginning to make sure your experience is smooth and stress-free.
Fast and Ultra-Sensitive Method for Analyzing Dopamine and Serotonin
In this application note, we introduce a fast and ultra-sensitive method for analyzing dopamine and serotonin levels with improved throughput and resolution time.
Specifications
HTEC-600 Specifications
| Detector | |
| Detection Methods | Amperometric Electrochemical Detector |
| Applied voltage | 0 – ±2,000ｍV, 1mV increment |
| Working Electrode Methods | Thin Layer Method |
| Working Electrode Size | Graphite (Standard), Pure Graphite, Grassy Carbon, Platinum, Gold, Silver |
| Working Electrode Gasket | Composed by TFE, Thickness 25 µm or 50 µm |
| Reference Electrode | Silver / Silver Chloride (Lithium Chloride Enclosed Type) |
| Auxiliary Electrode | SUS316 |
| Wetting Part Material | PEEK, SUS316 |
| Recorder Output Signal | ±10 V Analog (0.1 nA = 1 mV) |
| Time Constant | 1.0 sec, 1.5 sec, 3.0 sec |
| Signal Input |
Signal In: Detect more than 300 msec, a contact signal M. Injector Signal In: Detect 300 sec switching from open/close signal |
| External Device Control Signal |
0 sec: Output contact signal for1 sec at the same time as signal input. 3 sec: Output contact signal for 1 sec at 3 sec from signal input. 6 sec: Output contact signal for 1 sec at 6 sec from signal input. |
| Liquid Feeding Pump | |
| Liquid Delivery System | Tandem Two Pistons |
| Replacement Volume Per Stroke | 80 µL |
| Piston Material | Sapphire |
| Setting Flow Rate Range | 1 – 3000 µL/min |
| Stable Flow Rate Range | 100 – 2000 µL/min |
| Wetting Part Material | PEEK, Sapphire, Ruby, PTFE, PCTFE |
| Maximum Pressure | 20 MPa |
| Pulsating Reduction Mechanism | Automatic Pulsation Control |
| Degasser | |
| Methods | Continuous Vacuum Membrane Permeation Type |
| Number of Channels | 2 Channels |
| Internal Capacity |
Upper: Around 300 µL/channel Bottom: Around 7.5 mL/channel |
| Constant Temperature Bath | |
| Methods | Heating/Cooling with peltier element w/ air circulation |
| Setting Temp Range | 15 – 50 °C (1 °C increment) |
| Temp Control Accuracy | ± 0.1 °C |
| Unit Size | |
| Dimension | 290 (W) x 380 (D) x 435 (H) mm (Excluding protrusions) |
| Weight | 23.2 kg |
| Power Supply | AC 100 – 240 V 50/60 Hz 300 VA |
Anatomy of the HTEC-600
Eicom’s Innovative Cell Design
- Ultrasensitive, 30 fg of dopamine
- Graphite electrode often ready to use for < 30 mins after start-up
- No tools to access the working electrode
- Easy to clean or change the working electrode
The key to the sensitivity is the cell design. Eicom relies on a spring-loaded mechanism that always maintains the proper pressure. This structure allows the detector to quickly stabilize after the cell is opened and closed. Another benefit is that the working electrode can easily be accessed with no tools. In this way, the electrode can easily be wiped clean and reinstalled to ensure maximum sensitivity.
Video Resources
The [HTEC] has been fantastic, it’s run beautifully, and it’s super easy to use.
I like that all of my settings can be controlled right here on the screen, instead of having to make method files within the software and uploading them to the system – that’s just too much work.
HTEC User Publications
Differing Patterns of Diurnal Variation and Impact of Antacids on Levodopa Pharmacokinetics in Patients with Parkinson’s Disease.
NAGAI, M., TADA, S., YAMANISHI, Y., MIYAUE, N., & ANDO, R.
(2023). Rinsho yakuri/Japanese Journal of Clinical Pharmacology and Therapeutics, 54(1), 3-8.
Reduced serotonergic transmission alters sensitivity to cost and reward via 5-HT1A and 5-HT1B receptors in monkeys.
Hori, Y., Mimura, K., Nagai, Y., Hori, Y., Kumata, K., Zhang, M. R., … & Minamimoto, T.
(2023). bioRxiv, 2023-02.
Broad Serotonergic Actions of Vortioxetine as a Promising Avenue for the Treatment of L-DOPA-Induced Dyskinesia.
Budrow, C., Elder, K., Coyle, M., Centner, A., Lipari, N., Cohen, S., … & Bishop, C.
(2023). Cells, 12(6), 837.
Tryptophan Hydroxylase-2-Mediated Serotonin Biosynthesis Suppresses Cell Reprogramming into Pluripotent State.
Sinenko, S. A., Kuzmin, A. A., Skvortsova, E. V., Ponomartsev, S. V., Efimova, E. V., Bader, M., … & Tomilin, A. N.
(2023). International Journal of Molecular Sciences, 24(5), 4862.
Opposing effects of clozapine and brexpiprazole on β-aminoisobutyric acid: Pathophysiology of antipsychotics-induced weight gain.
Fukuyama, K., Motomura, E., & Okada, M.
(2023). Schizophrenia, 9(1), 8.
Let’s Get Started!
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