HPLC-ECD, HTEC-600

Start detecting neurotransmitters immediately

upon delivery with no prior knowledge

of liquid chromatography

Get Started

Most researcher’s first approach to neurotransmitter analysis can leave them feeling unsure where to start and overwhelmed. As a company founded and operated by scientific minds, we get it.

That is why we developed the HTEC-600, an HPLC-ECD (High Performance Liquid Chromatography – Electrochemical Detection).  The HTEC-600 offers pre-configured applications and protocols so your journey into neurotransmitter analysis is clear from start to finish.

All-in-One Turnkey HPLC-ECD

Avoid the potential confusion caused that comes from individually configuring the multiple components required for neurotransmitter analysis. The HTEC-600 combines all of the key components required for hyper sensitive neurotransmitter analysis into one easily configurable system so you can get started immediately from the moment it arrives in your lab. 

HTEC-600

” The [HTEC] has been fantastic, it’s run beautifully, and it’s super easy to use.

I like that all of my settings can be controlled right here on the screen, instead of having to make method files within the software and uploading them to the system – that’s just too much work. “

Sarah Ryan
Augusta University

Anatomy of the HPLC-ECD, HTEC-600

Electrochemical Detector

Electrochemical Detector

Amperometic Design with inexpensive and interchangeable working electrodes

Online Solvent Degasser

Online Solvent Degasser

For Enhanced Pump Reliability

HTEC-600-Open
Pulse Quenching Pump

Pulse Quenching Pump

Computer controlled and no pulse damper is required

Ultra-high Sensitivity with Ultra-low Requirements

Allow our specialists to do the hard work for you. Follow our pre-configured protocols and applications designed to provide you with a step-by-step guide to achieving the ideal conditions required to obtain the highest quality analysis of your sample type.

 

 

 

 

 

 

 

 

 

How to Get Started

Introducing our 3 step plan to make sure your journey into neurotransmitter analysis is clear from start to finish. 

 

 

Step 1

Download a free application note covering your ideal sample type and application



 

 

Step 2

Find the optimal conditions for your sample type with the help of our neurotransmitter analysis specialists! 

We will contact you shortly after downloading the application note in Step 1.

Can’t wait?

Call us now at 888-680-7775

 

 

 

Step 3

Begin neurotransmitter analysis with a clear direction and understanding of the process!

 

 

 

 

For a deeper understanding of HPLC-ECD fundamentals, explore our HPLC-ECD (Electrochemical Detection) Fundamentals page. Let’s embark on this journey together, unlocking the mysteries of neurotransmitter analysis with confidence.

HPLC-ECD, HTEC-600 Specifications

HTEC-600 Specifications
Detector
Detection MethodsAmperometric Electrochemical Detector
Applied voltage0 – ±2,000mV, 1mV increment
Working Electrode MethodsThin Layer Method
Working Electrode SizeGraphite (Standard), Pure Graphite, Grassy Carbon, Platinum, Gold, Silver
Working Electrode GasketComposed by TFE, Thickness 25 µm or 50 µm
Reference ElectrodeSilver / Silver Chloride (Lithium Chloride Enclosed Type)
Auxiliary ElectrodeSUS316
Wetting Part MaterialPEEK, SUS316
Recorder Output Signal±10 V Analog (0.1 nA = 1 mV)
Time Constant1.0 sec, 1.5 sec, 3.0 sec
Signal Input

Signal In: Detect more than 300 msec, a contact signal

M. Injector Signal In:  Detect 300 sec  switching from open/close signal

External Device Control Signal

0 sec: Output contact signal for1 sec  at the same time as signal input.

3 sec: Output contact signal for 1 sec at 3 sec from signal input.

6 sec: Output contact signal for 1 sec at 6 sec from signal input.

Liquid Feeding Pump
Liquid Delivery SystemTandem Two Pistons
Replacement Volume Per Stroke80 µL
Piston MaterialSapphire
Setting Flow Rate Range1 – 3000 µL/min
Stable Flow Rate Range100 – 2000 µL/min
Wetting Part MaterialPEEK, Sapphire, Ruby, PTFE, PCTFE
Maximum Pressure20 MPa
Pulsating Reduction MechanismAutomatic Pulsation Control
Degasser
MethodsContinuous Vacuum Membrane Permeation Type
Number of Channels2 Channels
Internal Capacity

Upper: Around 300 µL/channel

Bottom: Around 7.5 mL/channel

Constant Temperature Bath
MethodsHeating/Cooling with peltier element w/ air circulation
Setting Temp Range15 – 50 °C (1 °C increment)
Temp Control Accuracy± 0.1 °C
Unit Size
Dimension290 (W) x 380 (D) x 435 (H) mm (Excluding protrusions)
Weight23.2 kg
Power SupplyAC 100 – 240 V 50/60 Hz 300 VA

“I love the [HPLC-ECD HTEC] system, it runs super fast and it’s so quiet.”

Video Resources

Benefits using HPLC-ECD for neurotransmitter detection

HPLC-ECD HTEC User Publications

Differing Patterns of Diurnal Variation and Impact of Antacids on Levodopa Pharmacokinetics in Patients with Parkinson’s Disease.
NAGAI, M., TADA, S., YAMANISHI, Y., MIYAUE, N., & ANDO, R.
(2023).  Rinsho yakuri/Japanese Journal of Clinical Pharmacology and Therapeutics54(1), 3-8.

Reduced serotonergic transmission alters sensitivity to cost and reward via 5-HT1A and 5-HT1B receptors in monkeys.
Hori, Y., Mimura, K., Nagai, Y., Hori, Y., Kumata, K., Zhang, M. R., … & Minamimoto, T.
(2023).  bioRxiv, 2023-02.

Broad Serotonergic Actions of Vortioxetine as a Promising Avenue for the Treatment of L-DOPA-Induced Dyskinesia.
Budrow, C., Elder, K., Coyle, M., Centner, A., Lipari, N., Cohen, S., … & Bishop, C.
(2023).  Cells12(6), 837.

Tryptophan Hydroxylase-2-Mediated Serotonin Biosynthesis Suppresses Cell Reprogramming into Pluripotent State.
Sinenko, S. A., Kuzmin, A. A., Skvortsova, E. V., Ponomartsev, S. V., Efimova, E. V., Bader, M., … & Tomilin, A. N.
(2023).  International Journal of Molecular Sciences24(5), 4862.

Opposing effects of clozapine and brexpiprazole on β-aminoisobutyric acid: Pathophysiology of antipsychotics-induced weight gain.
Fukuyama, K., Motomura, E., & Okada, M.
(2023).  Schizophrenia9(1), 8.

Recent Blog Articles

Let’s Get Started!

Ask about our loaner program of the HTEC.