HPLC-ECD, HTEC-600
Start detecting neurotransmitters immediately upon delivery with no prior knowledge of liquid chromatography Get guidance on your application
Getting started with neurotransmitter analysis can feel unclear, from selecting the right method to ensuring reliable results.
The HTEC-600, developed by Eicom, simplifies this process with a guided, turnkey HPLC-ECD system that combines hardware, application support, and expert guidance from start to finish.
All-in-one turnkey HPLC-ECD
Avoid the potential confusion caused that comes from individually configuring the multiple components required for neurotransmitter analysis. The HTEC-600 combines all of the key components required for hyper sensitive neurotransmitter analysis into one easily configurable system so you can get started immediately from the moment it arrives in your lab.
What researchers say
“The [HTEC] has been fantastic, it’s run beautifully, and it’s super easy to use.
I like that all of my settings can be controlled right here on the screen, instead of having to make method files within the software and uploading them to the system – that’s just too much work.”
– Sarah Ryan, Augusta University
Anatomy of the HPLC-ECD, HTEC-600
Electrochemical Detector
Amperometic design
with inexpensive and interchangeable working electrodes
Online Solvent Degasser
For Enhanced Pump Reliability
Pulse Quenching Pump
Computer controlled and no pulse damper is required
Ultra-high sensitivity with
ultra-low requirements
The HTEC-600 is designed to deliver high-sensitivity detection with stable, repeatable performance.
Preconfigured hardware and application workflows reduce setup complexity, allowing you to focus on generating reliable data.
How to Get Started with HPLC-ECD
A clear, guided path from selecting the right application to running your first samples with confidence.
STEP 1
Download an application note
Get a starting point for your analyte and sample type, including recommended methods and conditions.
STEP 2
Tell us your sample and goals
Share your analyte and sample type, and our specialists will recommend the most appropriate application and setup for your workflow.
STEP 3
Installation and guided setup in your lab
One of our specialists travels to your lab to install the system, run standards, and ensure everything is working properly so your team can begin analysis with confidence.
For a deeper understanding of HPLC-ECD fundamentals, explore our fundamentals page.
Once your workflow is defined, explore system specifications and configuration details below.
HTEC-600 Specifications
| Detector | |
| Detection Methods | Amperometric Electrochemical Detector |
| Applied voltage | 0 – ±2,000ｍV, 1mV increment |
| Working Electrode Methods | Thin Layer Method |
| Working Electrode Size | Graphite (Standard), Pure Graphite, Grassy Carbon, Platinum, Gold, Silver |
| Working Electrode Gasket | Composed by TFE, Thickness 25 µm or 50 µm |
| Reference Electrode | Silver / Silver Chloride (Lithium Chloride Enclosed Type) |
| Auxiliary Electrode | SUS316 |
| Wetting Part Material | PEEK, SUS316 |
| Recorder Output Signal | ±10 V Analog (0.1 nA = 1 mV) |
| Time Constant | 1.0 sec, 1.5 sec, 3.0 sec |
| Signal Input | Signal In: Detect more than 300 msec, a contact signal M. Injector Signal In: Detect 300 sec switching from open/close signal |
| External Device Control Signal | 0 sec: Output contact signal for1 sec at the same time as signal input. 3 sec: Output contact signal for 1 sec at 3 sec from signal input. 6 sec: Output contact signal for 1 sec at 6 sec from signal input. |
| Liquid Feeding Pump | |
| Liquid Delivery System | Tandem Two Pistons |
| Replacement Volume Per Stroke | 80 µL |
| Piston Material | Sapphire |
| Setting Flow Rate Range | 1 – 3000 µL/min |
| Stable Flow Rate Range | 100 – 2000 µL/min |
| Wetting Part Material | PEEK, Sapphire, Ruby, PTFE, PCTFE |
| Maximum Pressure | 20 MPa |
| Pulsating Reduction Mechanism | Automatic Pulsation Control |
| Degasser | |
| Methods | Continuous Vacuum Membrane Permeation Type |
| Number of Channels | 2 Channels |
| Internal Capacity | Upper: Around 300 µL/channel Bottom: Around 7.5 mL/channel |
| Constant Temperature Bath | |
| Methods | Heating/Cooling with peltier element w/ air circulation |
| Setting Temp Range | 15 – 50 °C (1 °C increment) |
| Temp Control Accuracy | ± 0.1 °C |
| Unit Size | |
| Dimension | 290 (W) x 380 (D) x 435 (H) mm (Excluding protrusions) |
| Weight | 23.2 kg |
| Power Supply | AC 100 – 240 V 50/60 Hz 300 VA |
Case Studies
Studying Pancreatic Islet Cell Biology with HPLC-ECD
Studying pancreatic islet cell biology uncovers GABA’s role in diabetes. HPLC-ECD enables precise GABA measurement, aiding research. The researcher discusses challenges, praising HTEC’s sensitivity and ease. Improved maintenance and increased sensitivity are desired.
Exploring Novel Compounds for Suppressing Drug Self-Administration
The Hemby Lab at High Point University utilizes HPLC-ECD analysis to explore novel compounds for mitigating drug self-administration in substance abuse disorder research, aiming to alleviate human suffering and societal burden.
Recent Blog Articles
Understanding and Preventing Baseline Drift in HPLC-ECD
Insights and Troubleshooting Tips from 30 Years of Experience High-performance liquid chromatography with electrochemical detection (HPLC-ECD) generally has one or two of the following detector modes: Amperometric DC, Coulometric DC, and Pulsed Amperometric Detection...
HPLC-ECD vs. LC-MS/MS: Which Is Best for Neurotransmitter Analysis?
Analyzing neurotransmitters in microdialysis or brain tissue samples often comes down to choosing the right analytical technique. Two popular options are HPLC-ECD and LC-MS/MS. Each offers clear advantages depending on your lab’s goals, budget, and detection needs....
Simultaneous Analysis of Glutamate and GABA for Neuroscience Applications
In this video, we’ll show you how to detect and analyze Glutamate and GABA in brain tissue or microdialysis samples in just 12 minutes.
HPLC-ECD HTEC User Publications
Differing Patterns of Diurnal Variation and Impact of Antacids on Levodopa Pharmacokinetics in Patients with Parkinson’s Disease.
NAGAI, M., TADA, S., YAMANISHI, Y., MIYAUE, N., & ANDO, R.
(2023). Rinsho yakuri/Japanese Journal of Clinical Pharmacology and Therapeutics, 54(1), 3-8.
Reduced serotonergic transmission alters sensitivity to cost and reward via 5-HT1A and 5-HT1B receptors in monkeys.
Hori, Y., Mimura, K., Nagai, Y., Hori, Y., Kumata, K., Zhang, M. R., … & Minamimoto, T.
(2023). bioRxiv, 2023-02.
Broad Serotonergic Actions of Vortioxetine as a Promising Avenue for the Treatment of L-DOPA-Induced Dyskinesia.
Budrow, C., Elder, K., Coyle, M., Centner, A., Lipari, N., Cohen, S., … & Bishop, C.
(2023). Cells, 12(6), 837.
Tryptophan Hydroxylase-2-Mediated Serotonin Biosynthesis Suppresses Cell Reprogramming into Pluripotent State.
Sinenko, S. A., Kuzmin, A. A., Skvortsova, E. V., Ponomartsev, S. V., Efimova, E. V., Bader, M., … & Tomilin, A. N.
(2023). International Journal of Molecular Sciences, 24(5), 4862.
Opposing effects of clozapine and brexpiprazole on β-aminoisobutyric acid: Pathophysiology of antipsychotics-induced weight gain.
Fukuyama, K., Motomura, E., & Okada, M.
(2023). Schizophrenia, 9(1), 8.
Let’s Get Started!
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