HPLC-ECD APPLICATION MANUAL
Monoamine analysis in tissue homogenate samples
Download the HPLC-ECD application manual for monoamine analysis in tissue homogenate samples, including dopamine, serotonin, norepinephrine, and related metabolites.
Use this manual to review the standard monoamine analysis workflow and evaluate whether HTEC-600 or Amuza sample analysis services are a fit for your lab.
Download the application manual
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WHAT YOU’LL FIND
What the application manual covers
The application manual outlines the standard HPLC-ECD workflow for monoamine analysis in tissue homogenate samples.
Monoamines and metabolites
Review the standard workflow for MHPG, NE, E, DOPAC, NM, DA, 5-HIAA, ISO, HVA, 3-MT, and 5-HT.
Sample preparation workflow
See the tissue homogenate preparation steps used before HPLC-ECD analysis.
Analytical conditions
Review the HPLC-ECD setup, column, flow rate, electrode, applied potential, and temperature.
Sample chromatograms
View representative chromatograms from standard solution and rat brain-region samples.
NEXT STEPS
Need monoamine data? Choose the path that fits your lab.
Whether you are evaluating an HPLC-ECD system for ongoing neurotransmitter analysis or need help running samples, Amuza can help you determine the best next step.
Bring HPLC-ECD into your lab
For labs that want to run neurotransmitter analysis in-house, the HTEC-600 supports HPLC-ECD workflows for high-sensitivity detection with application support.
Explore HTEC-600Have Amuza run your samples
For labs that need preliminary data, have a backlog of samples, or want help with analysis before investing in equipment, Amuza offers neurotransmitter detection and analysis services.
Request Sample AnalysisLooking for L-DOPA-related monoamine analysis? Amuza can help you determine which application manual fits your workflow.