TeleFiOpto combines wireless fiber photometry recording and optogenetic stimulation in one head-mounted system. Record fluorescence signals while delivering light stimulation to the targeted neural population during the same behavioral experiment.
Connect neural activity with optogenetic stimulation
TeleFiOpto combines wireless fiber photometry and optogenetics in one head-mounted system. Record fluorescence signals while delivering light stimulation to the targeted neural population during the same behavioral experiment.
Record and stimulate simultaneously
Monitor activity-related fluorescence signals while delivering optogenetic stimulation through the same headstage and implanted fiber.
Support freely moving behavior
Remove the long optical tether typically used for fiber photometry and optogenetics, reducing restrictions on movement during behavioral experiments.
Connect signals with experimental events
Compare recorded neural activity with stimulation periods and behavioral events throughout the experiment.
Wireless recording and stimulation through one headstage
TeleFiOpto combines two wireless communication paths within one experimental setup. Fiber photometry data is transmitted from the headstage to the receiver, while optogenetic stimulation commands are sent to the headstage through infrared communication.
Photometry data transmitted over the 2.4 GHz band
Stimulation commands transmitted through infrared communication
Connect the headstage
Attach the TeleFiOpto headstage to the implanted fiber cannula at the target site.
Record fluorescence signals
Transmit fiber photometry data wirelessly to the TeleFipho receiver and recording software.
Deliver optical stimulation
Program stimulation settings and send commands to the headstage through the infrared emitter.
Compare activity and events
Review fluorescence activity alongside stimulation periods and behavioral events.
Record photometry signals alongside experimental events
TeleFiOpto records fluorescence signals while optogenetic stimulation and behavioral events occur during the experiment. Researchers can review changes in neural activity in relation to defined stimulation periods and experimental events.
View fluorescence signals during recording
Monitor the raw photometry trace as data is collected from the wireless headstage.
Track stimulation and event timing
Record stimulation periods and experimental events alongside the fluorescence signal for easier comparison.
Prepare data for further analysis
Review recorded signals and event timing using the supplied TeleFipho software and analysis workflow.
Match TeleFiOpto to your experiment
TeleFiOpto is designed for experiments that combine GFP-like fluorescent indicators with yellow- to red-sensitive optogenetic actuators. Compatibility depends on the spectral properties of the selected indicator and opsin, the target region, and the implanted fiber cannula.
Fluorescent indicators
Manufacturer examples of compatible GFP-like indicators include:
- GCaMP
- dLight
- GRAB-DA
Optogenetic actuators
Manufacturer examples of yellow- to red-sensitive actuators include:
- NpHR
- Chrimson
- Jaws
- rsChRmine
Fiber cannula selection
Fiber cannulas are available with 200, 400, or 600 µm core diameters.
A 600 µm cannula is recommended for optogenetic stimulation. The 200 µm cannula is not recommended for this application.
Not sure whether your indicator and opsin are compatible?
Tell us about your experimental setup and we can help you determine the appropriate TeleFiOpto configuration.
Record GCaMP activity during optogenetic stimulation
In this manufacturer-provided example, TeleFiOpto was used to record GCaMP fluorescence while stimulating rsChRmine expressed in the same neuronal population. Neural activity was monitored before, during, and after optical stimulation through the same headstage and implanted fiber.
Example data courtesy of Dr. Masanori Nomoto, Inokuchi Lab, Toyama University, and provided by Bio Research Center Co., Ltd. Experimental conditions and responses may vary according to the indicator, opsin, target region, and stimulation protocol.
Choose the right TeleFiOpto configuration
TeleFiOpto is available as a complete system or as an expansion package for laboratories that already use TeleFipho or Teleopto components.
TeleFiOpto complete set
For laboratories starting with wireless fiber photometry and optogenetics
Includes the TeleFiOpto headstage, fiber photometry receiver and accessories, recording software, infrared stimulation components, remote controller, and optogenetics stimulator.
TeleFiOpto Opto Pack
For laboratories that already have a TeleFipho system
Adds the components needed to introduce optogenetic stimulation, including the TeleFiOpto headstage and stimulation-control equipment.
TeleFiOpto Fipho Pack
For laboratories that already have a Teleopto system
Adds the fiber photometry receiver, recording software, headstage, charging equipment, cannulas, and implantation accessories needed for fluorescence recording.
Package contents and required accessories depend on your existing equipment and selected cannula configuration.
Compare included components
Compare the components included with the complete TeleFiOpto system and the two expansion packages.
| Component | Complete set | Opto Pack | Fipho Pack |
|---|---|---|---|
| TeleFiOpto headstage TeleFiOpHS | 1 Included, quantity 1 | 1 Included, quantity 1 | 1 Included, quantity 1 |
| TeleFipho receiver TeleFiR | 1 Included, quantity 1 | Not included | 1 Included, quantity 1 |
| TeleFipho charger TeleFiCharger | 1 Included, quantity 1 | Not included | 1 Included, quantity 1 |
| TeleFipho fiber cannulas TeleFiC-l-d | 3 Included, quantity 3 | Not included | 3 Included, quantity 3 |
| Insertion tool TeleFiTool | 1 Included, quantity 1 | Not included | 1 Included, quantity 1 |
| Dummy headstage TeleFiDummy | 1 Included, quantity 1 | Not included | 1 Included, quantity 1 |
| TeleFipho software | 1 Included, quantity 1 | Not included | 1 Included, quantity 1 |
| pMAT Converter software | 1 Included, quantity 1 | Not included | 1 Included, quantity 1 |
| Teleopto remote controller TeleRemocon | 1 Included, quantity 1 | 1 Included, quantity 1 | Not included |
| Infrared emitter TeleEmitter | 1 Included, quantity 1 | 1 Included, quantity 1 | Not included |
| Optogenetics stimulator STOmk-2 | 1 Included, quantity 1 | 1 Included, quantity 1 | Not included |
The complete set and Fipho Pack include three fiber cannulas. Cannula length and core diameter must be specified when ordering.
A 5 mm length and 400 µm core will be supplied when no cannula configuration is specified. A 600 µm core is recommended for optogenetic stimulation.
Need help identifying the correct package?
Tell us which TeleFipho or Teleopto components you already use, and our team can help determine the appropriate configuration.
Technical specifications
Review the recording, wireless communication, stimulation, and fiber cannula specifications for the TeleFiOpto system.
Headstage and fiber photometry
| Headstage weight | 3.5 g |
|---|---|
| Headstage dimensions | 12 × 12 × 23 mm |
| Excitation wavelength | LED peak: 470 nm Filter band: 445–490 nm |
| Emission wavelength | Filter band: 500–550 nm |
| Excitation power | 5–200 µW at the end of a 400 µm fiber; adjustable |
| Sampling rate | 100 Hz |
| A/D resolution | 16 bit |
| Photosensor | Photodiode |
| Gain | 1010 V/A |
Wireless communication and software
| Battery duration | Approximately 2 hours at 30 µW excitation power |
|---|---|
| Transmission band | 2.4 GHz |
| Transmission distance | Up to 2 m |
| Power | Rechargeable battery |
| Receiver input | 1 general-purpose analog input, 0–5 V |
| Receiver output | 1 photometry analog output |
| PC connection | USB |
| Software | TeleFipho software and pMAT Converter software |
Optogenetic stimulation and fiber cannula
| Stimulation wavelength | LED peak: 620 nm Filter band: 600–640 nm |
|---|---|
| Maximum stimulation power | 6 mW/mm² at the end of a 600 µm fiber; adjustable |
| Cannula core diameters | 200, 400, or 600 µm |
| Numerical aperture | 0.39 |
| Ferrule diameter | 2.5 mm |
| Cannula length | Specified when ordering |
| Recommended for stimulation | 600 µm core cannula |
Specifications are subject to change. Cannula length and core diameter must be specified when ordering.
Frequently asked questions
Learn more about TeleFiOpto compatibility, system configuration, recording, and optogenetic stimulation.
Can TeleFiOpto record and stimulate at the same time?
Yes. TeleFiOpto is designed to record fiber photometry signals while delivering optogenetic stimulation through the same headstage and implanted fiber cannula.
Which fluorescent indicators can be used with TeleFiOpto?
Manufacturer examples include GFP-like fluorescent indicators such as GCaMP, dLight, and GRAB-DA. Compatibility depends on the spectral properties of the selected indicator and the complete experimental configuration.
Which optogenetic actuators are compatible?
TeleFiOpto is designed for use with yellow- to red-sensitive optogenetic actuators. Manufacturer examples include NpHR, Chrimson, Jaws, and rsChRmine.
Which fiber cannula should I select?
Fiber cannulas are available with 200, 400, or 600 µm core diameters. The manufacturer recommends the 600 µm cannula for optogenetic stimulation and does not recommend the 200 µm cannula for that application.
Cannula length and core diameter must be specified when ordering.
Can TeleFiOpto be added to an existing TeleFipho system?
Yes. The TeleFiOpto Opto Pack is intended for laboratories that already use TeleFipho and need the additional components required for optogenetic stimulation.
Can TeleFiOpto be added to an existing Teleopto system?
Yes. The TeleFiOpto Fipho Pack adds the fiber photometry headstage, receiver, software, charging equipment, cannulas, and related accessories to an existing Teleopto setup.
How is recording data transmitted?
Fiber photometry data is transmitted wirelessly from the headstage to the TeleFipho receiver over the 2.4 GHz band. The receiver connects to the recording computer through USB.
How are optogenetic stimulation commands delivered?
Stimulation settings are programmed through the Teleopto stimulation components. Commands are then transmitted to the TeleFiOpto headstage through infrared communication.
How long does the TeleFiOpto headstage battery last?
The published battery duration is approximately two hours when operating at 30 µW excitation power. Actual duration may vary according to the experimental settings.
Can Amuza help confirm whether TeleFiOpto fits my experiment?
Yes. Share your fluorescent indicator, optogenetic actuator, target region, cannula requirements, and current TeleFipho or Teleopto equipment with our team. We can help identify the appropriate system configuration for your study.
Resources for planning your experiment
Review the TeleFiOpto brochure and explore practical guides for planning wireless fiber photometry and optogenetics experiments.
TeleFiOpto brochure
Review the system configuration, package contents, example data, and ordering information.
Wireless fiber photometry guide
Learn about system setup, signal recording, experimental planning, and considerations for freely moving studies.
Wireless optogenetics guide
Review key considerations for optical stimulation, experimental design, and wireless behavioral research.
Combining fiber photometry and optogenetics
Explore how simultaneous recording and stimulation can connect neural activity with experimental intervention.
Planning a wireless recording and stimulation experiment?
Tell us about your fluorescent indicator, optogenetic actuator, target region, and current equipment. Our team can help determine whether TeleFiOpto and the available system configuration fit your study.