Wireless Fiber Photometry
Obtain precise real-time measurements of neural circuits during operant conditioning.
Fiber photometry is a powerful ultra-fast technique used to measure calcium, neurotransmitters, and other molecules in vivo. To use fiber photometry, genetically encoded calcium indicators (GECIs), dopamine indicators (DLIGHT1), norepinephrine indicators (GRABNE), and other fluorescent sensors are first expressed at the location of interest. An optical fiber is then used to excite the sensor and detect the resulting fluorescence.
But when used in freely moving animals, a long optical cable (patch cord) attached to the head of the animal can be problematic. The cable can impede movement in environments containing exercise wheels or shelters, and also alter social interactions. The cable can also cause artifacts when used with video tracking systems. For example, the cable often continues to sway after the animal has stopped moving, making it difficult for software to recognize freezing behavior. Placing all components on a headstage ends all of these problems.
TeleFipho includes all of the components required for fiber photometry – optical fiber, filter cube, light source, and photodetector – as well as wireless transmission hardware, all in a 3-gram headstage.
TeleFipho definitely will not block the free behavior of your animals, enabling novel experimental approaches using fiber photometry.
Features
- World-first commercial wireless fiber photometry
- Small headstage / good for mice, rats, marmosets, etc.
- Standard 2.5 mm ferrule cannula
- Rechargeable by a dedicated charger
- Adjustable excitation LED power
- Adjustable signal offset
- For GCaMP or GFP-like indicators
- Half the price of traditional fiber photometry systems!
Simple setup – headstage, receiver and software
TeleFipho has been tested with both mice and rats. The data above shows stress-induced (tail pinch) changes in GCaMP signals from hypothalamic orexin neurons in mice. GCaMP is a genetically encoded calcium indicator often used to monitor calcium dynamics. Data is Courtesy of Dr. Daisuke Ono in the Akihiro Yamanaka Lab, Nagoya University.
Our lab collected its first tiny bit of fiber photometry data this week (using Amuza wireless), and I am pleased as punch! We were so excited to get data so quickly.
PETH was generated using pMAT software created by David Barker at Rutgers University
TeleFipho Fiber Photometry Standard Set
- TeleFiT 1x TeleFipho Transmitter Headstage
- TeleFiR 1x TeleFipho Receiver
- TeleFiCharger 1x TeleFipho Charger
- TeleFiC_x 3x TeleFipho Cannula
- TeleFiTool 1x Insertion Tool for TeleFipho
- TeleFiDummy 1x TeleFipho Dummy Headstage
- 1x TeleFipho software installer
(※) Please specify cannula length.
Download the FREE eBook!
Fiber photometry is one of the newest tools available to neuroscientists to help correlate behavior with neural activity.
This ebook introduces topics and references critical for using fiber photometry during behavioral experiments
Stand Alone / External Recorder
Data can be directly read from Matlab / Octave or can be exported to ASCII format.
For more complicated online analysis and/or parallel recording of other physiological data, you can access photometry data as an analog signal via BNC on the front panel of the TeleFipho receiver so that you can connect the external data recording system (e.g. PowerLab from ADI).
Publication Highlight
Cardenas A, Papadogiannis A, Dimitrov E. The role of medial prefrontal cortex projections to locus ceruleus in mediating the sex differences in behavior in mice with inflammatory pain. FASEB J. 2021 Jul;35(7):e21747. doi: 10.1096/fj.202100319RR. PMID: 34151467; PMCID: PMC8283812.
Animals: male and female mice
Vector: pAAV5.Syn.GCaMP6f, pAAV5.Syn.GCaMP6f.WPRE.SV40
Sensor: GCaMP6f
Target Region: right mPFC (medial prefrontal cortex)
Coordinates: 1.8, ±0.4, and −2.2 mm in respect to bregma
Fiber: fiber core 400 μm NA 0.39, length 3 mm
Behavior test: Elevated O-maze
Fiber photometry data analysis: Amuza TeleFipho software
Model: Injection of complete Freund’s adjuvant (CFA) as a model for inflammatory pain
Results: Inflammatory pain altered the both the behavior and the activity of the mPFC ([Ca2+] as measured by fiber photometry) of male but not female mice.
Telefipho Fiber Photometry Specifications
| Headstage Weight | 3 g |
| Headstage size | 12 x 12 x 22 mm |
| Excitation wavelength | LED peak 470 nm, Filter band 445～490 nm |
| Emission wavelength | Filter band 500～550 nm |
| Excitation power | 10～300 µW ＠ Fiber end (Adjustable) |
| Sampling rate | 100Hz |
| AD resolution | 16 bit |
| Photosensor | Photodiode |
| Gain | 1010 V/A |
| Battery life | 2 hours ＠ Excitation power 30 µW |
| Transmission band | 2.4 GHz |
| Transmission distance | 2 m |
| Power | Battery-powered, rechargeable |
| Receiver I/O | 1x Photometry analog out, 1x General purpose analog In (-2.5~5V) |
| PC Interface | USB / TeleFipho software (for Windows 10) |
| Cannula | core: 400 µm / NA 0.39, Cladding: 425 µm, Ferrule: 2.5 mm |
| Model | Description |
| TeleFipho-set | TeleFipho Standard Set |
| TeleFiT | TeleFipho Transmitter Headstage |
| TeleFiR | TeleFipho Receiver |
| TeleFiCharger | TeleFipho Charger |
| TeleFiC_x | TeleFipho Cannula |
| TeleFiTool | Insertion Tool for TeleFipho |
| TeleFiDummy | TeleFipho Dummy Headstage |
Frequently Asked Questions
What is fiber photometry?
Fiber Photometry is an ultra-fast fluorescence technique for monitoring neurochemical levels in vivo in real-time.
What is needed to perform fiber photometry?
Fiber photometry systems require a light source, fluorescence detector, filters, fiber optics, and software for signal processing. The first three are typically separate components but are also available combined as a wireless headstage. Fiber photometry systems can be integrated with behavioral equipment, electrophysiology, video capture, and other systems to create an integrated timeline of behavioral and brain events.
What can I measure using fiber photometry?
Calcium, as well as many neurotransmitters (Dopamine, serotonin, norepinephrine, acetylcholine), amino acids (glutamate, GABA) and other molecules (ATP, adenosine, endocannabinoids) can all be measured in vivo using fiber photometry. New sensors are constantly being invented, so this list is rapidly growing.
Do I have to use a patch cord with in vivo fiber photometry?
No! The Amuza TeleFipho system uses a rechargeable wireless headstage and doesn’t need a patchcord. This makes it an excellent choice for behavioral experiments.
What is the isosbestic control signal in fiber photometry?
The Isos signal provides a way to separate [calcium] (or another analyte) dependent changes in your data (the part you want) from calcium-independent changes. The independent changes can include bending/kinking of a patchcord, rotary joint and other connection faults, ambient light changes, and autofluorescence. Amuza wireless fiber photometry has no patchcord and only one ferrule/sleeve connection, making it possible to use fiber photometry without an isos signal.
How large or deep an area can be probed by fiber photometry?
The Pisanello lab found that 80% of the fluorescent signal was collected from within 200 μm of the tip of the fiber. This was determined with a 200 μm core fiber; with larger fibers, the collection area can be much larger.