Wireless Fiber Photometry

Obtain precise real-time measurements of neural circuits during operant conditioning.

Fiber photometry is a powerful ultra-fast technique used to measure calcium, neurotransmitters, and other molecules in vivo. To use fiber photometry, genetically encoded calcium indicators (GECIs), dopamine indicators (DLIGHT1), norepinephrine indicators (GRABNE), and other fluorescent sensors are first expressed at the location of interest. An optical fiber is then used to excite the sensor and detect the resulting fluorescence.

But when used in freely moving animals, a long optical cable (patch cord) attached to the head of the animal can be problematic. The cable can impede movement in environments containing exercise wheels or shelters, and also alter social interactions. The cable can also cause artifacts when used with video tracking systems. For example, the cable often continues to sway after the animal has stopped moving, making it difficult for software to recognize freezing behavior. Placing all components on a headstage ends all of these problems.

Download the FREE Fiber Photometry eBook!

Telefipho Fiber Photometry transmitter headstage

 

TeleFipho includes all of the components required for fiber photometry – optical fiber, filter cube, light source, and photodetector – as well as wireless transmission hardware, all in a 3-gram headstage.

TeleFipho fiber photometry systems definitely will not block the free behavior of your animals, enabling novel experimental approaches using fiber photometry.

Telefipho Wireless Fiber Photometry
My lab has been using TeleFipho wireless photometric system for the past two years. The system is simple to use, durable and reliable. The practicality of TeleFipho allowed us to collect in vivo data about the neuronal activity of various limbic regions of the CNS during behavioral tests in mice.
Eugene Dimitrov MD, PhD

Assistant Professor, Department of Physiology and Biophysics Center for the Neurobiology of Stress Resilience and Psychiatric Disorders, Rosalind Franklin University of Medicine and Science

Telefipho Fiber Photometry Diagram

Features

  • World-first commercial wireless fiber photometry
  • Small headstage / good for mice, rats, marmosets, etc.
  • Standard 2.5 mm ferrule cannula
  • Rechargeable by a dedicated charger
  • Adjustable excitation LED power
  • Adjustable signal offset
  • For GCaMP or GFP-like indicators
  • Half the price of traditional fiber photometry systems!
Telefipho Fiber Photometry Simple Setup - headstage, receiver and software

Simple setup – headstage, receiver and software

Telefipho Fiber Photometry gCaMP signal graph

The TeleFipho fiber photometry system has been tested with both mice and rats. The data above shows stress-induced (tail pinch) changes in GCaMP signals from hypothalamic orexin neurons in mice. GCaMP is a genetically encoded calcium indicator often used to monitor calcium dynamics. Data is Courtesy of Dr. Daisuke Ono in the Akihiro Yamanaka Lab, Nagoya University.

Our lab collected its first tiny bit of fiber photometry data this week (using Amuza wireless), and I am pleased as punch! We were so excited to get data so quickly.

Prof. Rebecca M. Shansky

Department of Psychology, Northeastern University

Tone Delta

PETH was generated using pMAT software created by David Barker at Rutgers University

TeleFipho Fiber Photometry Standard Set

TeleFipho Fiber Photometry Standard Set consists of the following items:

  • TeleFiT 1x TeleFipho Transmitter Headstage
  • TeleFiR 1x TeleFipho Receiver
  • TeleFiCharger 1x TeleFipho Charger
  • TeleFiC_x 3x TeleFipho Cannula
  • TeleFiTool 1x Insertion Tool for TeleFipho
  • TeleFiDummy 1x TeleFipho Dummy Headstage
  • 1x TeleFipho software installer

(※) Please specify cannula length.

Download the FREE eBook!

Fiber photometry is one of the newest tools available to neuroscientists to help correlate behavior with neural activity.

This ebook introduces topics and references critical for using fiber photometry during behavioral experiments

fiber photometry

Stand Alone / External Recorder

Telefipho Fiber Photometry stand alone external recorder
TeleFipho receiver can be directly connected to PC via USB for fiber photometry data recording in TeleFipho software. TeleFipho software is good enough for simple raw data / low pass filtered data recording.

Data can be directly read from Matlab / Octave or can be exported to ASCII format.
For more complicated online analysis and/or parallel recording of other physiological data, you can access photometry data as an analog signal via BNC on the front panel of the TeleFipho receiver so that you can connect the external data recording system (e.g. PowerLab from ADI).

Publication Highlight

Cardenas A, Papadogiannis A, Dimitrov E. The role of medial prefrontal cortex projections to locus ceruleus in mediating the sex differences in behavior in mice with inflammatory pain. FASEB J. 2021 Jul;35(7):e21747. doi: 10.1096/fj.202100319RR. PMID: 34151467; PMCID: PMC8283812.

Animals: male and female mice
Vector: pAAV5.Syn.GCaMP6f, pAAV5.Syn.GCaMP6f.WPRE.SV40

Sensor: GCaMP6f
Target Region: right mPFC (medial prefrontal cortex)
Coordinates: 1.8, ±0.4, and −2.2 mm in respect to bregma
Fiber: fiber core 400 μm NA 0.39, length 3 mm
Behavior test: Elevated O-maze
Fiber photometry data analysis: Amuza TeleFipho software

Model: Injection of complete Freund’s adjuvant (CFA) as a model for inflammatory pain

Results: Inflammatory pain altered the both the behavior and the activity of the mPFC ([Ca2+] as measured by fiber photometry) of male but not female mice.

Telefipho Fiber Photometry Specifications

Headstage Weight 3 g
Headstage size 12 x 12 x 22 mm
Excitation wavelength LED peak 470 nm, Filter band 445~490 nm
Emission wavelength Filter band 500~550 nm
Excitation power 10~300 µW @ Fiber end (Adjustable)
Sampling rate 100Hz
AD resolution 16 bit
Photosensor Photodiode
Gain 1010 V/A
Battery life 2 hours @ Excitation power 30 µW
Transmission band 2.4 GHz
Transmission distance 2 m
Power Battery-powered, rechargeable
Receiver I/O 1x Photometry analog out, 1x General purpose analog In (-2.5~5V)
PC Interface USB / TeleFipho software (for Windows 10)
Cannula core: 400 µm / NA 0.39, Cladding: 425 µm, Ferrule: 2.5 mm
Model Description
TeleFipho-set TeleFipho Standard Set
TeleFiT TeleFipho Transmitter Headstage
TeleFiR TeleFipho Receiver
TeleFiCharger TeleFipho Charger
TeleFiC_x TeleFipho Cannula
TeleFiTool Insertion Tool for TeleFipho
TeleFiDummy TeleFipho Dummy Headstage
TeleFipho

Frequently Asked Questions

What is fiber photometry?

Fiber Photometry is an ultra-fast fluorescence technique for monitoring neurochemical levels in vivo in real-time.

What is needed to perform fiber photometry?

Fiber photometry systems require a light source, fluorescence detector, filters, fiber optics, and software for signal processing. The first three are typically separate components but are also available combined as a wireless headstage. Fiber photometry systems can be integrated with behavioral equipment, electrophysiology, video capture, and other systems to create an integrated timeline of behavioral and brain events.

What can I measure using fiber photometry?

Calcium, as well as many neurotransmitters (Dopamine, serotonin, norepinephrine, acetylcholine), amino acids (glutamate, GABA) and other molecules (ATP, adenosine, endocannabinoids) can all be measured in vivo using fiber photometry. New sensors are constantly being invented, so this list is rapidly growing.

Do I have to use a patch cord with in vivo fiber photometry?

No! The Amuza TeleFipho system uses a rechargeable wireless headstage and doesn’t need a patchcord. This makes it an excellent choice for behavioral experiments.

What is the isosbestic control signal in fiber photometry?

The Isos signal provides a way to separate [calcium] (or another analyte) dependent changes in your data (the part you want) from calcium-independent changes. The independent changes can include bending/kinking of a patchcord, rotary joint and other connection faults, ambient light changes, and autofluorescence. Amuza wireless fiber photometry has no patchcord and only one ferrule/sleeve connection, making it possible to use fiber photometry without an isos signal.

How large or deep an area can be probed by fiber photometry?

The Pisanello lab found that 80% of the fluorescent signal was collected from within 200 μm of the tip of the fiber. This was determined with a 200 μm core fiber; with larger fibers, the collection area can be much larger.

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