Wireless Fiber Photometry
Precise, real-time neural measurements in freely moving animals.
Why Fiber Photometry Matters
Fiber photometry is a powerful technique for studying neural activity in vivo. By pairing fluorescent sensors such as GCaMP calcium indicators or neurotransmitter probes with behavioral experiments, researchers can directly observe how brain circuits drive behavior.
Traditional fiber photometry systems rely on long patch cords that:
Restrict movement and social interaction.
Introduce motion artifacts and electrical noise.
Complicate setups, limiting scalability and reproducibility
The TeleFipho Wireless Fiber Photometry system overcomes these barriers. Compact and cable-free, it enables researchers to correlate neural signals with natural, unrestricted behavior.
Enhanced Mobility
Animals can move freely without the constraints of patch cords, allowing for more natural behaviors and interactions.
Reliable Data Collection
The system minimizes artifacts, ensuring accurate data capture even in complex environments.
User-Friendly Setup
The all-in-one headstage simplifies the setup process, making it accessible for labs of all sizes.
Download the FREE eBook!
Fiber photometry is one of the newest tools available to neuroscientists to help correlate behavior with neural activity.
This ebook introduces topics and references critical for using fiber photometry during behavioral experiments
The TeleFipho system includes all necessary components for fiber photometry, integrated into a 3-gram headstage. This design ensures that the system does not interfere with the free behavior of your animals, enabling innovative experimental approaches.
Testimonials
Intravenous self-administration and tethered photometry have not been reliably done. It’s a great testament to what the system can do and how it can be implemented in a way that overcomes challenges with tethered photometry.
My lab has been using TeleFipho wireless photometric system for the past two years. The system is simple to use, durable and reliable. The practicality of TeleFipho allowed us to collect in vivo data about the neuronal activity of various limbic regions of the CNS during behavioral tests in mice.
Our lab collected its first tiny bit of fiber photometry data this week (using Amuza wireless), and I am pleased as punch! We were so excited to get data so quickly.
PETH was generated using pMAT software created by David Barker at Rutgers University
Product Features
- World-first commercial wireless fiber photometry
- Suitable for various animals (mice, rats, marmosets, etc.)
- Standard 2.5 mm ferrule cannula
- Rechargeable with a dedicated charger
- Adjustable excitation LED power
- Adjustable signal offset
- Compatible with GCaMP or GFP-like indicators
- Cost-effective: Half the price of traditional systems
Research Data and Publications
Simple setup – headstage, receiver and software
The TeleFipho system has been tested with both mice and rats. The data below shows stress-induced changes in GCaMP signals from hypothalamic orexin neurons in mice.
Data courtesy of Dr. Daisuke Ono in the Akihiro Yamanaka Lab, Nagoya University.
Publication Highlight
Cardenas A, Papadogiannis A, Dimitrov E. The role of medial prefrontal cortex projections to locus ceruleus in mediating the sex differences in behavior in mice with inflammatory pain. FASEB J. 2021 Jul;35(7):e21747. doi: 10.1096/fj.202100319RR. PMID: 34151467; PMCID: PMC8283812.
Animals: male and female mice
Vector: pAAV5.Syn.GCaMP6f, pAAV5.Syn.GCaMP6f.WPRE.SV40
Sensor: GCaMP6f
Target Region: right mPFC (medial prefrontal cortex)
Coordinates: 1.8, ±0.4, and −2.2 mm in respect to bregma
Fiber: fiber core 400 μm NA 0.39, length 3 mm
Behavior test: Elevated O-maze
Fiber photometry data analysis: Amuza TeleFipho software
Model: Injection of complete Freund’s adjuvant (CFA) as a model for inflammatory pain
Results: Inflammatory pain altered the both the behavior and the activity of the mPFC ([Ca2+] as measured by fiber photometry) of male but not female mice.
Fiber photometry
2026
Activity dynamics in the NPY neuronal signaling of mPFC in response to an air puff.
Dimitrov, E., Usdin, T., & Urban, J. H.
(2026). Stress, 29(1), 2666067.
2025
Teneurin-4 knockdown disrupts dopamine dynamics and attenuates methamphetamine-induced behaviors.
Chen, W., Yokose, J., Izuo, N., Yano, Y., Kaigawa, T., Kai, N., ... & Nitta, A.
(2025). Neuropharmacology, 110817.
Hypothalamic Prostaglandins Facilitate Recovery From Severe Hypoglycemia but Exacerbate Recurrent Hypoglycemia in Mice.
Abe, T., Xu, S., Sugiura, Y., Arima, Y., Hayasaka, T., Lee, M. L., ... & Toda, C.
(2025). Diabetes, 74(12), 2390-2404.
Peripheral opioid receptor antagonism alleviates fentanyl-induced cardiorespiratory depression and is devoid of aversive behavior.
Ruyle, B. C., Masud, S., Kesaraju, R., Tahirkheli, M., Modh, J., Roth, C. G., ... & Morón, J. A.
(2025). eLife, 13, RP104469.
Striosome circuitry stimulation inhibits striatal dopamine release and locomotion.
Okunomiya, T., Watanabe, D., Banno, H., Kondo, T., Imamura, K., Takahashi, R., & Inoue, H.
(2025). Journal of Neuroscience, 45(4).
Peripheral opioid receptor antagonism alleviates fentanyl-induced cardiorespiratory depression and is devoid of aversive behavior.
Ruyle, B. C., Masud, S., Kesaraju, R., Tahirkheli, M., Modh, J., Roth, C. G., ... & Morón, J. A.
(2025). eLife, 13, RP104469.
Estradiol protects against pain-facilitated fentanyl use via suppression of opioid-evoked dopamine activity in males.
Higginbotham, J. A., Abt, J. G., Teich, R. H., Dearman, J. J., Lintz, T., & Morón, J. A.
(2025). Neuron.
Mouse CA1 vasoactive intestinal polypeptide-expressing interneurons as novelty detectors: functional investigation using wireless techniques.
Tamboli, S.
(2025). Thesis/Dissertation
2024
Altered firing output of VIP interneurons and early dysfunctions in CA1 hippocampal circuits in the 3xTg mouse model of Alzheimer’s disease.
Michaud, F., Francavilla, R., Topolnik, D., Iloun, P., Tamboli, S., Calon, F., & Topolnik, L.
(2024). Elife, 13, RP95412.
Individual Differences in Dopamine Signaling in a Rodent Model of Substance Use Disorder Vulnerability
Leach, A. C.
(2024). (Doctoral dissertation, Wake Forest University).
cognitive deficits in social isolation-reared mice.
Yokoyama, R., Ago, Y., Igarashi, H., Higuchi, M., Tanuma, M., Shimazaki, Y., ... & Hashimoto, H.
(2024).
Rapid adaptation to spatial goals through activation of the ventral tegmental area-hippocampal dopaminergic pathway.
Tamatsu, Y.
(2024). Doctoral Dissertation , 1–85.
Striatal cholinergic transmission in an inducible transgenic mouse model of paroxysmal non-kinesiogenic dyskinesia.
Scarduzio, M., Jaunarajs, K. L. E., & Standaert, D. G.
(2024). Neurobiology of Disease, 201, 106685.
Protocol for synchronized wireless fiber photometry and video recordings in rodents during behavior.
Tamboli, S., Topolnik, D., Radhakrishnan, R., Veilleux-Lemieux, D., & Topolnik, L.
(2024). STAR Protocols, 5(4), 103407.
Signal flow in the NMDA receptor–dependent phosphoproteome regulates postsynaptic plasticity for aversive learning.
Funahashi, Y., Ahammad, R. U., Zhang, X., Hossen, E., Kawatani, M., Nakamuta, S., ... & Kaibuchi, K.
(2024). Science Signaling, 17(853), eado9852
Brain-implantable needle-type CMOS imaging device enables multi-layer dissection of seizure calcium dynamics in the hippocampus.
Olorocisimo, J. P., Ohta, Y., Regonia, P. R., Castillo, V. C. G., Yoshimoto, J., Takehara, H., ... & Ohta, J.
(2024). Journal of Neural Engineering.
Mouse hippocampal CA1 VIP interneurons detect novelty in the environment and support recognition memory.
Tamboli, S., Singh, S., Topolnik, D., Barkat, M. E. A., Radhakrishnan, R., Guet-McCreight, A., & Topolnik, L.
(2024). Cell Reports, 43(4).
(R)-ketamine restores anterior insular cortex activity and cognitive deficits in social isolation-reared mice.
Yokoyama, R., Ago, Y., Igarashi, H., Higuchi, M., Tanuma, M., Shimazaki, Y., ... & Hashimoto, H.
(2024). Molecular Psychiatry, 1-11.
2023
Alcohol inhibits sociability via serotonin inputs to the nucleus accumbens.
Marcinkiewcz, C., Wang, R., Khan, K., Balasubramanian, N., James, T., Pushpavathi, S., ... & Hefti, M.
(2023). Research Square, rs-3.
Optogenetic activation of the ventral tegmental area-hippocampal pathway facilitates rapid adaptation to changes in spatial goals.
Tamatsu, Y., Azechi, H., Takahashi, R., Sawatani, F., Ide, K., Fujiyama, F., & Takahashi, S.
(2023). Iscience, 26(12).
Fiber Photometry: A novel methodology for in vivo calcium imaging in anterior cingulate cortex (ACC) to ventrolateral periaqueductal grey (vlPAG) projecting neurons during fear conditioning.
Hulshof, L. G.
(2023).
NMDAR Phosphoproteome Controls Synaptic Growth and Learning.
Funahashi, Y., Ahammad, R. U., Zhang, X., Hossen, E., Kawatani, M., Nakamuta, S., ... & Kaibuchi, K.
(2023). bioRxiv, 2023-12.
2022
Time-dependent enhancement in ventral tegmental area dopamine neuron activity drives pain-facilitated fentanyl intake in males.
Higginbotham, J. A., Abt, J. G., Tiech, R. H., & Morón, J. A.
(2022). bioRxiv, 2022-08.
Locus Coeruleus-Noradrenergic Neurons Regulate Stress Coping During Subchronic Exposure to Social Threats: A Characteristic Feature in Postpartum Female Mice.
Nakamura, A., Muroi, Y., & Ishii, T.
(2022). Cellular and Molecular Neurobiology, 1-18.
Deconstruction of a hypothalamic astrocyte-white adipocyte sympathetic axis that regulates lipolysis in mice.
Chen, D., Qi, Y., Zhang, J., & Yang, Y.
(2022). Nature Communications, 13(1), 1-16.
A possible mechanism for development of working memory impairment in male mice subjected to inflammatory pain.
Papadogiannis, A., & Dimitrov, E.
(2022). Neuroscience.
Enhanced motor cortex output and disinhibition in asymptomatic female mice with C9orf72 genetic expansion.
Amalyan, S., Tamboli, S., Lazarevich, I., Topolnik, D., Bouman, L. H., & Topolnik, L.
(2022). Cell Reports, 40(1), 111043.
2021
HCN2 in cholinergic interneurons of the nucleus accumbens mediates reward response.
Lee, J., Weinberger, M., Kawahara, Y., Cheng, J., Umschweif, G., Medrihan, L., ... & Sagi, Y.
(2021). bioRxiv, 2021-09.
Activation of septal OXTr neurons induces anxiety-but not depressive-like behaviors.
Huang, T., Guan, F., Licinio, J., Wong, M. L., & Yang, Y.
(2021). Molecular Psychiatry, 1-10.
The role of medial prefrontal cortex projections to locus ceruleus in mediating the sex differences in behavior in mice with inflammatory pain.
Cardenas, A., Papadogiannis, A., & Dimitrov, E.
(2021). The FASEB Journal, 35(7), e21747.
Product Specifications
| Headstage Weight | 3 g |
| Headstage size | 12 x 12 x 22 mm |
| Excitation wavelength | LED peak 470 nm, Filter band 445～490 nm |
| Emission wavelength | Filter band 500～550 nm |
| Excitation power | 10～300 µW ＠ Fiber end (Adjustable) |
| Sampling rate | 100Hz |
| AD resolution | 16 bit |
| Photosensor | Photodiode |
| Gain | 1010 V/A |
| Battery life | 2 hours ＠ Excitation power 30 µW |
| Transmission band | 2.4 GHz |
| Transmission distance | 2 m |
| Power | Battery-powered, rechargeable |
| Receiver I/O | 1x Photometry analog out, 1x General purpose analog In (-2.5~5V) |
| PC Interface | USB / TeleFipho software (for Windows 10) |
| Cannula | core: 400 µm / NA 0.39, Cladding: 425 µm, Ferrule: 2.5 mm |
| Model | Description |
| TeleFipho-set | TeleFipho Standard Set |
| TeleFiT | TeleFipho Transmitter Headstage |
| TeleFiR | TeleFipho Receiver |
| TeleFiCharger | TeleFipho Charger |
| TeleFiC_x | TeleFipho Cannula |
| TeleFiTool | Insertion Tool for TeleFipho |
| TeleFiDummy | TeleFipho Dummy Headstage |
Stand Alone / External Recorder
TeleFipho receiver can be directly connected to a PC via USB for data recording using TeleFipho software. For more complex analysis or parallel recording of other physiological data, photometry data can be accessed via BNC on the receiver’s front panel.
Frequently Asked Questions
What is fiber photometry?
Fiber Photometry is an ultra-fast fluorescence technique for monitoring neurochemical levels in vivo in real-time.
What is needed to perform fiber photometry?
Fiber photometry systems require a light source, fluorescence detector, filters, fiber optics, and software for signal processing. The first three are typically separate components but are also available combined as a wireless headstage. Fiber photometry systems can be integrated with behavioral equipment, electrophysiology, video capture, and other systems to create an integrated timeline of behavioral and brain events.
What can I measure using fiber photometry?
Calcium, as well as many neurotransmitters (Dopamine, serotonin, norepinephrine, acetylcholine), amino acids (glutamate, GABA) and other molecules (ATP, adenosine, endocannabinoids) can all be measured in vivo using fiber photometry. New sensors are constantly being invented, so this list is rapidly growing.
Do I have to use a patch cord with in vivo fiber photometry?
No! The Amuza TeleFipho system uses a rechargeable wireless headstage and doesn’t need a patchcord. This makes it an excellent choice for behavioral experiments.
What is the isosbestic control signal in fiber photometry?
The Isos signal provides a way to separate [calcium] (or another analyte) dependent changes in your data (the part you want) from calcium-independent changes. The independent changes can include bending/kinking of a patchcord, rotary joint and other connection faults, ambient light changes, and autofluorescence. Amuza wireless fiber photometry has no patchcord and only one ferrule/sleeve connection, making it possible to use fiber photometry without an isos signal.
How large or deep an area can be probed by fiber photometry?
The Pisanello lab found that 80% of the fluorescent signal was collected from within 200 μm of the tip of the fiber. This was determined with a 200 μm core fiber; with larger fibers, the collection area can be much larger.
Recent Blog Articles
Combining Wireless Optogenetics and Fiber Photometry for Closed-Loop Experiments
Optogenetic stimulation and fiber photometric recording have played crucial, yet distinct, roles in in vivo behavioral neuroscience. These tools allow researchers to directly link the activity of specific cell types and brain regions to observable behaviors, helping...
Fiber Photometry: An Introductory Guide to This Revolutionary Technique, Part 3
Discover the TeleFipho Wireless Fiber Photometry System, liberating research animals for unrestricted movement. Amuza Inc’s comprehensive eBook delves into this revolutionary tool, paving the way for breakthroughs in neuroscience research methodologies.
Fiber Photometry: An Introductory Guide to This Revolutionary Technique, Part 2
Explore the intricate components of Fiber Photometry, from light sources to genetic indicators. Discover how Amuza Inc’s innovations revolutionize this neuroscience technique for groundbreaking research advancements.