Wireless Fiber Photometry

Obtain precise real-time measurements of neural circuits during operant conditioning.

Fiber photometry is a powerful ultra-fast technique used to measure calcium, neurotransmitters, and other molecules in vivo. To use fiber photometry, genetically encoded calcium indicators (GECIs), dopamine indicators (DLIGHT1), norepinephrine indicators (GRABNE), and other fluorescent sensors are first expressed at the location of interest. An optical fiber is then used to excite the sensor and detect the resulting fluorescence.

But when used in freely moving animals, a long optical cable (patch cord) attached to the head of the animal can be problematic. The cable can impede movement in environments containing exercise wheels or shelters, and also alter social interactions. The cable can also cause artifacts when used with video tracking systems. For example, the cable often continues to sway after the animal has stopped moving, making it difficult for software to recognize freezing behavior. Placing all components on a headstage ends all of these problems.

Telefipho Fiber Photometry transmitter headstage
 

TeleFipho includes all of the components required for fiber photometry – optical fiber, filter cube, light source, and photodetector – as well as wireless transmission hardware, all in a 3-gram headstage.

TeleFipho definitely will not block the free behavior of your animals, enabling novel experimental approaches using fiber photometry.

My lab has been using TeleFipho wireless photometric system for the past two years. The system is simple to use, durable and reliable. The practicality of TeleFipho allowed us to collect in vivo data about the neuronal activity of various limbic regions of the CNS during behavioral tests in mice.
Eugene Dimitrov MD, PhD

Assistant Professor, Department of Physiology and Biophysics Center for the Neurobiology of Stress Resilience and Psychiatric Disorders, Rosalind Franklin University of Medicine and Science

Telefipho Fiber Photometry Diagram

Features

  • World-first commercial wireless fiber photometry
  • Small headstage / good for mice, rats, marmosets, etc.
  • Standard 2.5 mm ferrule cannula
  • Rechargeable by a dedicated charger
  • Adjustable excitation LED power
  • Adjustable signal offset
  • For GCaMP or GFP-like indicators
  • Half the price of traditional fiber photometry systems!
Telefipho Fiber Photometry Simple Setup - headstage, receiver and software

Simple setup – headstage, receiver and software

Telefipho Fiber Photometry gCaMP signal graph

TeleFipho has been tested with both mice and rats. The data above shows stress-induced (tail pinch) changes in GCaMP signals from hypothalamic orexin neurons in mice. GCaMP is a genetically encoded calcium indicator often used to monitor calcium dynamics. Data is Courtesy of Dr. Daisuke Ono in the Akihiro Yamanaka Lab, Nagoya University.

Our lab collected its first tiny bit of fiber photometry data this week (using Amuza wireless), and I am pleased as punch! We were so excited to get data so quickly.

Prof. Rebecca M. Shansky

Department of Psychology, Northeastern University

PETH was generated using pMAT software created by David Barker at Rutgers University

TeleFipho Fiber Photometry Standard Set

TeleFipho Standard Set consists of the following items:

  • TeleFiT 1x TeleFipho Transmitter Headstage
  • TeleFiR 1x TeleFipho Receiver
  • TeleFiCharger 1x TeleFipho Charger
  • TeleFiC_x 3x TeleFipho Cannula
  • TeleFiTool 1x Insertion Tool for TeleFipho
  • TeleFiDummy 1x TeleFipho Dummy Headstage
  • 1x TeleFipho software installer

(※) Please specify cannula length.

Stand Alone / External Recorder

Telefipho Fiber Photometry stand alone external recorder
TeleFipho receiver can be directly connected to PC via USB for fiber photometry data recording in TeleFipho software. TeleFipho software is good enough for simple raw data / low pass filtered data recording.

Data can be directly read from Matlab / Octave or can be exported to ASCII format.
For more complicated online analysis and/or parallel recording of other physiological data, you can access photometry data as an analog signal via BNC on the front panel of the TeleFipho receiver so that you can connect the external data recording system (e.g. PowerLab from ADI).

Telefipho Fiber Photometry Specifications

Headstage Weight 3 g
Headstage size12 x 12 x 22 mm
Excitation wavelengthLED peak 470 nm, Filter band 445~490 nm
Emission wavelengthFilter band 500~550 nm
Excitation power10~300 µW @ Fiber end (Adjustable)
Sampling rate100Hz
AD resolution16 bit
PhotosensorPhotodiode
Gain1010 V/A
Battery life2 hours @ Excitation power 30 µW
Transmission band2.4 GHz
Transmission distance2 m
PowerBattery-powered, rechargeable
Receiver I/O1x Photometry analog out, 1x General purpose analog In (-2.5~5V)
PC InterfaceUSB / TeleFipho software (for Windows 10)
Cannulacore: 400 µm / NA 0.39, Cladding: 425 µm, Ferrule: 2.5 mm
ModelDescription
TeleFipho-setTeleFipho Standard Set
TeleFiTTeleFipho Transmitter Headstage
TeleFiRTeleFipho Receiver
TeleFiChargerTeleFipho Charger
TeleFiC_xTeleFipho Cannula
TeleFiToolInsertion Tool for TeleFipho
TeleFiDummyTeleFipho Dummy Headstage

Let’s Get Started!

Virtual demos available.