Wireless Fiber Photometry
Obtain precise real-time measurements of neural circuits during operant conditioning.
Fiber photometry is a powerful ultra-fast technique used to measure calcium, neurotransmitters, and other molecules in vivo. To use fiber photometry, genetically encoded calcium indicators (GECIs), dopamine indicators (DLIGHT1), norepinephrine indicators (GRABNE), and other fluorescent sensors are first expressed at the location of interest. An optical fiber is then used to excite the sensor and detect the resulting fluorescence.
But when used in freely moving animals, a long optical cable (patch cord) attached to the head of the animal can be problematic. The cable can impede movement in environments containing exercise wheels or shelters, and also alter social interactions. The cable can also cause artifacts when used with video tracking systems. For example, the cable often continues to sway after the animal has stopped moving, making it difficult for software to recognize freezing behavior. Placing all components on a headstage ends all of these problems.
TeleFipho includes all of the components required for fiber photometry – optical fiber, filter cube, light source, and photodetector – as well as wireless transmission hardware, all in a 3-gram headstage.
TeleFipho definitely will not block the free behavior of your animals, enabling novel experimental approaches using fiber photometry.
My lab has been using TeleFipho wireless photometric system for the past two years. The system is simple to use, durable and reliable. The practicality of TeleFipho allowed us to collect in vivo data about the neuronal activity of various limbic regions of the CNS during behavioral tests in mice.
- World-first commercial wireless fiber photometry
- Small headstage / good for mice, rats, marmosets, etc.
- Standard 2.5 mm ferrule cannula
- Rechargeable by a dedicated charger
- Adjustable excitation LED power
- Adjustable signal offset
- For GCaMP or GFP-like indicators
- Half the price of traditional fiber photometry systems!
TeleFipho Standard Set
TeleFipho Standard Set consists of the following items：
- TeleFiT 1x TeleFipho Transmitter Headstage
- TeleFiR 1x TeleFipho Receiver
- TeleFiCharger 1x TeleFipho Charger
- TeleFiC_x 3x TeleFipho Cannula
- TeleFiTool 1x Insertion Tool for TeleFipho
- TeleFiDummy 1x TeleFipho Dummy Headstage
- 1x TeleFipho software installer
(※) Please specify cannula length.
Stand Alone / External Recorder
TeleFipho receiver can be directly connected to PC via USB for fiber photometry data recording in TeleFipho software. TeleFipho software is good enough for simple raw data / low pass filtered data recording.
Data can be directly read from Matlab / Octave or can be exported to ASCII format.
For more complicated online analysis and/or parallel recording of other physiological data, you can access photometry data as an analog signal via BNC on the front panel of the TeleFipho receiver so that you can connect the external data recording system (e.g. PowerLab from ADI).
|Headstage Weight||3 g|
|Headstage size||12 x 12 x 22 mm|
|Excitation wavelength||LED peak 470 nm, Filter band 445～490 nm|
|Emission wavelength||Filter band 500～550 nm|
|Excitation power||10～300 µW ＠ Fiber end (Adjustable)|
|AD resolution||16 bit|
|Battery life||2 hours ＠ Excitation power 30 µW|
|Transmission band||2.4 GHz|
|Transmission distance||2 m|
|Receiver I/O||1x Photometry analog out, 1x General purpose analog In (0~5V)|
|PC Interface||USB / TeleFipho software (for Windows 10)|
|Cannula||core: 400 µm / NA 0.39, Cladding: 425 µm, Ferrule: 2.5 mm|
|TeleFipho-set||TeleFipho Standard Set|
|TeleFiT||TeleFipho Transmitter Headstage|
|TeleFiTool||Insertion Tool for TeleFipho|
|TeleFiDummy||TeleFipho Dummy Headstage|