Wireless Fiber Photometry

Precise, real-time neural measurements in freely moving animals.

Fiber photometry is a powerful technique for measuring calcium, neurotransmitters, and other molecules in vivo. Using genetically encoded calcium indicators (GECIs) and other fluorescent sensors, researchers can gain insights into neural activity. Traditional systems, however, often require long optical cables that can impede animal movement and introduce artifacts. The TeleFipho Wireless Fiber Photometry system overcomes these limitations, offering a comprehensive, cable-free solution.

  • Compact and lightweight: At just 3 grams, it doesn’t hinder animal movement.
  • All-in-one design: Includes optical fiber, filter cube, light source, and photodetector.
  • Wireless freedom: No long cables to restrict or interfere with behavior.

Enhanced Mobility

Animals can move freely without the constraints of patch cords, allowing for more natural behaviors and interactions.

Reliable Data Collection

The system minimizes artifacts, ensuring accurate data capture even in complex environments.

User-Friendly Setup

The all-in-one headstage simplifies the setup process, making it accessible for labs of all sizes.

The TeleFipho system includes all necessary components for fiber photometry, integrated into a 3-gram headstage. This design ensures that the system does not interfere with the free behavior of your animals, enabling innovative experimental approaches.

Telefipho Wireless Fiber Photometry

Testimonials

My lab has been using TeleFipho wireless photometric system for the past two years. The system is simple to use, durable and reliable. The practicality of TeleFipho allowed us to collect in vivo data about the neuronal activity of various limbic regions of the CNS during behavioral tests in mice.

Eugene Dimitrov MD, PhD

Assistant Professor, Department of Physiology and Biophysics Center for the Neurobiology of Stress Resilience and Psychiatric Disorders, Rosalind Franklin University of Medicine and Science

Our lab collected its first tiny bit of fiber photometry data this week (using Amuza wireless), and I am pleased as punch! We were so excited to get data so quickly.

Prof. Rebecca M. Shansky

Department of Psychology, Northeastern University

Tone Delta

PETH was generated using pMAT software created by David Barker at Rutgers University

Telefipho Fiber Photometry Diagram

Product Features

  • World-first commercial wireless fiber photometry
  • Suitable for various animals (mice, rats, marmosets, etc.)
  • Standard 2.5 mm ferrule cannula
  • Rechargeable with a dedicated charger
  • Adjustable excitation LED power
  • Adjustable signal offset
  • Compatible with GCaMP or GFP-like indicators
  • Cost-effective: Half the price of traditional systems

Research Data and Publications

Telefipho Fiber Photometry Simple Setup - headstage, receiver and software

Simple setup – headstage, receiver and software

The TeleFipho system has been tested with both mice and rats. The data below shows stress-induced changes in GCaMP signals from hypothalamic orexin neurons in mice.

Telefipho Fiber Photometry gCaMP signal graph

Data courtesy of Dr. Daisuke Ono in the Akihiro Yamanaka Lab, Nagoya University.

Publication Highlight

Cardenas A, Papadogiannis A, Dimitrov E. The role of medial prefrontal cortex projections to locus ceruleus in mediating the sex differences in behavior in mice with inflammatory pain. FASEB J. 2021 Jul;35(7):e21747. doi: 10.1096/fj.202100319RR. PMID: 34151467; PMCID: PMC8283812.

Animals: male and female mice
Vector: pAAV5.Syn.GCaMP6f, pAAV5.Syn.GCaMP6f.WPRE.SV40

Sensor: GCaMP6f
Target Region: right mPFC (medial prefrontal cortex)
Coordinates: 1.8, ±0.4, and −2.2 mm in respect to bregma
Fiber: fiber core 400 μm NA 0.39, length 3 mm
Behavior test: Elevated O-maze
Fiber photometry data analysis: Amuza TeleFipho software

Model: Injection of complete Freund’s adjuvant (CFA) as a model for inflammatory pain

Results: Inflammatory pain altered the both the behavior and the activity of the mPFC ([Ca2+] as measured by fiber photometry) of male but not female mice.

Product Specifications

Headstage Weight 3 g
Headstage size12 x 12 x 22 mm
Excitation wavelengthLED peak 470 nm, Filter band 445~490 nm
Emission wavelengthFilter band 500~550 nm
Excitation power10~300 µW @ Fiber end (Adjustable)
Sampling rate100Hz
AD resolution16 bit
PhotosensorPhotodiode
Gain1010 V/A
Battery life2 hours @ Excitation power 30 µW
Transmission band2.4 GHz
Transmission distance2 m
PowerBattery-powered, rechargeable
Receiver I/O1x Photometry analog out, 1x General purpose analog In (-2.5~5V)
PC InterfaceUSB / TeleFipho software (for Windows 10)
Cannulacore: 400 µm / NA 0.39, Cladding: 425 µm, Ferrule: 2.5 mm
ModelDescription
TeleFipho-setTeleFipho Standard Set
TeleFiTTeleFipho Transmitter Headstage
TeleFiRTeleFipho Receiver
TeleFiChargerTeleFipho Charger
TeleFiC_xTeleFipho Cannula
TeleFiToolInsertion Tool for TeleFipho
TeleFiDummyTeleFipho Dummy Headstage
TeleFipho

Download the FREE eBook!

Fiber photometry is one of the newest tools available to neuroscientists to help correlate behavior with neural activity.

This ebook introduces topics and references critical for using fiber photometry during behavioral experiments

fiber photometry

Stand Alone / External Recorder

Telefipho Fiber Photometry stand alone external recorder

TeleFipho receiver can be directly connected to a PC via USB for data recording using TeleFipho software. For more complex analysis or parallel recording of other physiological data, photometry data can be accessed via BNC on the receiver’s front panel.

 

Frequently Asked Questions

What is fiber photometry?

Fiber Photometry is an ultra-fast fluorescence technique for monitoring neurochemical levels in vivo in real-time.

What is needed to perform fiber photometry?

Fiber photometry systems require a light source, fluorescence detector, filters, fiber optics, and software for signal processing. The first three are typically separate components but are also available combined as a wireless headstage. Fiber photometry systems can be integrated with behavioral equipment, electrophysiology, video capture, and other systems to create an integrated timeline of behavioral and brain events.

What can I measure using fiber photometry?

Calcium, as well as many neurotransmitters (Dopamine, serotonin, norepinephrine, acetylcholine), amino acids (glutamate, GABA) and other molecules (ATP, adenosine, endocannabinoids) can all be measured in vivo using fiber photometry. New sensors are constantly being invented, so this list is rapidly growing.

Do I have to use a patch cord with in vivo fiber photometry?

No! The Amuza TeleFipho system uses a rechargeable wireless headstage and doesn’t need a patchcord. This makes it an excellent choice for behavioral experiments.

What is the isosbestic control signal in fiber photometry?

The Isos signal provides a way to separate [calcium] (or another analyte) dependent changes in your data (the part you want) from calcium-independent changes. The independent changes can include bending/kinking of a patchcord, rotary joint and other connection faults, ambient light changes, and autofluorescence. Amuza wireless fiber photometry has no patchcord and only one ferrule/sleeve connection, making it possible to use fiber photometry without an isos signal.

How large or deep an area can be probed by fiber photometry?

The Pisanello lab found that 80% of the fluorescent signal was collected from within 200 μm of the tip of the fiber. This was determined with a 200 μm core fiber; with larger fibers, the collection area can be much larger.

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