Microresico® Low-Bind 96-well Plate Minimizes binding

Microresico® Low-Bind 96-well Plate

$278.30

40 plates/pack

Minimize Protein or Peptide Binding

The number of analytes handled in biochemistry is becoming smaller. Thus, it is critical to reducing the loss of protein or peptide, caused by their binding to the polypropylene wall.

While standard hydrophilicity treatment prevents such binding, it is unfavorable in recovering samples.

Low protein binding
Low elution because of no coating on the material
High fluid recovery
350 µL capacity per well
V-shaped bottom
Autoclavable (121°C for 20 min.)

SKU: RC9.201.80 Category: Labware

Description

LOW BINDING
Microresico® features a low level of peptide or protein absorption to the container wall with no coating.

FLUID RECOVERY
With Microresico®, you can maximize the recovery of fluid samples.

Peptide Binding Analyses using HPLC

Method

1) Incubate each tube with Trypsin-digested BSA for 24h.
2) Collect and concentrate the unbound peptides.
3) Dissolve each concentrate with 0.1% TFA/H2O.
4) Analyze by Nano RP-HPLC.
Control: Trypsin-digested BSA diluted with 0.1 TFA/H2O.

Protein Binding Analyses using ELISA

Method

1) Incubate each tube with Adiponectin (0.75 ng/mL in PBS) for 3h.
2) Quantify the unbound adiponectin by ELISA to determine the loss rate.

Fluid Recovery through Pipetting

Method

1) Incubate each tube with dye-solution (Bordeaux S) for 1h.
2) Remove the dye solution from the tube by pipetting, add 0.5ml distilled water to the tube, and analyze the absorption of the dye to determine the residual rate.

Specifications

Material: Polypropylene, Non-sterile
Dimension: 86 x 128 x 14.4 mm
Bottom: V-Shaped
Autoclavable: 121℃ 20 min

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Microresico® Low-Bind 96-well Plate